Intracellular Detection and Localization of Nanoparticles by Refractive Index Measurement
Alain Géloën,
Karyna Isaieva,
Mykola Isaiev,
Olga Levinson,
Emmanuelle Berger,
Vladimir Lysenko
Affiliations
Alain Géloën
UMR Ecologie Microbienne Lyon (LEM), CNRS 5557, INRAE 1418, Claude Bernard University of Lyon, VetAgro Sup, Research Team “Bacterial Opportunistic Pathogens and Environment” (BPOE), University of Lyon, F-69622 Villeurbanne, France
Karyna Isaieva
IADI, INSERM, Université de Lorraine, F-54000 Nancy, France
Mykola Isaiev
LEMTA, CNRS, Université de Lorraine, F-54000 Nancy, France
Olga Levinson
Ray Techniques LTD, P.O. Box 39162, Jerusalem 9139101, Israel
Emmanuelle Berger
UMR Ecologie Microbienne Lyon (LEM), CNRS 5557, INRAE 1418, Claude Bernard University of Lyon, VetAgro Sup, Research Team “Bacterial Opportunistic Pathogens and Environment” (BPOE), University of Lyon, F-69622 Villeurbanne, France
Vladimir Lysenko
Light Matter Institute, UMR-5306, Claude Bernard University of Lyon, 2 rue Victor Grignard, F-69622 Villeurbanne, France
The measuring of nanoparticle toxicity faces an important limitation since it is based on metrics exposure, the concentration at which cells are exposed instead the true concentration inside the cells. In vitro studies of nanomaterials would benefit from the direct measuring of the true intracellular dose of nanoparticles. The objective of the present study was to state whether the intracellular detection of nanodiamonds is possible by measuring the refractive index. Based on optical diffraction tomography of treated live cells, the results show that unlabeled nanoparticles can be detected and localized inside cells. The results were confirmed by fluorescence measurements. Optical diffraction tomography paves the way to measuring the true intracellular concentrations and the localization of nanoparticles which will improve the dose-response paradigm of pharmacology and toxicology in the field of nanomaterials.
