Morphofunctional status of stem cells culture on 2D matrix imitating «quiescent» osteogenic and hemopoietic microterritories

Abstract

An influence relief features and quantitative parameters of model bone mineral matrix on in vitro morphofunctional status of human prenatal stromal lung cells (HPSLC) and in vivo mice bone/marrow system remodeling has been studied. Investigations have showed that magnetron calcium phosphate (CaP) coatings are thin (thickness less than 1—2 μm), nonporous, smooth (Ra < 1 μm) layer of carboxylated apatites with the calcium/phosphorus ratio higher than stoichiometric one (more than 1.67). Such 2D surfaces have discreet structure-functional microterritories (niches) for HPSLC. Nevertheless, HPSLC maturation in osteoblasts under 4-days cultivation on such surfaces is unlikely. Poor HPSLC osteogenic committing on «smooth» (Ra < 1 μm) CaP coating is in vitro associated with an average index of artificial osteogenic niche less than 34%, weak artificial surface dissolution and elevated TNFα secretion by cell culture. A behavior of such implants with singenic bone marrow column is displayed in vivo by a degeneration of mice heterotopic osteogenesis and hemopoiesis. Methodology suggested allows apparently to imitate bone surface microterritories having “quiescent“ endosteal niches for stromal and hemopoietic stem cells.

Keywords

• имплантаты
• кальций-фосфатные покрытия
• ниша
• краткосрочная культура клеток человека
• щелочная и кислая фосфатазы
• гетеротопический тест
• мыши