Sitafloxacin Expresses Potent Anti-Mycobacterium abscessus Activity

Siyuan He; Siyuan He; Qi Guo; Qi Guo; Lan Zhao; Liyun Xu; Junsheng Fan; Junsheng Fan; Wenye Wu; Zhemin Zhang; Bing Li; Haiqing Chu; Haiqing Chu

doi:10.3389/fmicb.2021.779531

Frontiers in Microbiology (Jan 2022)

Sitafloxacin Expresses Potent Anti-Mycobacterium abscessus Activity

Siyuan He,
Siyuan He,
Qi Guo,
Qi Guo,
Lan Zhao,
Liyun Xu,
Junsheng Fan,
Junsheng Fan,
Wenye Wu,
Zhemin Zhang,
Bing Li,
Haiqing Chu,
Haiqing Chu

Affiliations

Siyuan He: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Siyuan He: School of Medicine, Tongji University, Shanghai, China
Qi Guo: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Qi Guo: School of Medicine, Tongji University, Shanghai, China
Lan Zhao: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Liyun Xu: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Junsheng Fan: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Junsheng Fan: School of Medicine, Tongji University, Shanghai, China
Wenye Wu: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Zhemin Zhang: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Bing Li: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Haiqing Chu: Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Haiqing Chu: Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China

DOI: https://doi.org/10.3389/fmicb.2021.779531
Journal volume & issue: Vol. 12

Abstract

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Therapeutic options for treating Mycobacterium abscessus infections are extremely limited; quinolones are important. The in vitro anti-M. abscessus activities of nine quinolones, emphasizing sitafloxacin, were investigated. Antimicrobial susceptibility testing was performed on 10 non-tuberculous mycobacterium reference strains and 194 clinical, M. abscessus isolates. The activity of sitafloxacin against intracellular M. abscessus residing within macrophages was also evaluated. A checkerboard assay was conducted to determine synergy between sitafloxacin and 10 clinically important antibiotics. Among the nine quinolones tested, sitafloxacin exhibited the greatest anti-M. abscessus activity with MIC50 and MIC90 of 1 and 2 mg/L, respectively. Sitafloxacin exerted a bacteriostatic effect on M. abscessus and inhibited the intracellular growth of M. abscessus at concentrations equivalent to clarithromycin. No antagonism between sitafloxacin and 10 clinically important anti-M. abscessus antibiotics was evident. In summary, sitafloxacin exhibited a significant advantage relative to other quinolones in inhibiting the growth of M. abscessus in vitro, suggesting the potential inclusion of sitafloxacin in new strategies to treat M. abscessus infections.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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