EVALUATION PHYTOCHEMISTRY, CYTOTOXIC ANALYSIS AND ANTIMICROBIAL GROSS ETHANOL EXTRACT OF LEAVES <i>Annona muricata</i> L. (ANNONACEAE)

Abstract

The leaves of Annona muricata L. are widely used medicinally, against parasitic diseases, including helminths, dysentery, malaria and related symptoms. The Amapá population use as analgesics and against urinary infections. This research aimed to perform the phytochemical screening, analyze cytotoxic activity and antibacterial of the crude ethanol extract of leaves Annona muricata L. By the method described by Esteban et al. were detected classes: alkaloids, steroids and triterpenoids. Cytotoxicity was analyzed against the Artemia salina, by the method of Mayer with some adaptations, it was tested crude extract concentrations of leaves of 50µg/mL, 100µg/mL, 250µg/mL, 500µg/mL, 750µg/mL, 1000µg/mL, observed toxicity at concentrations from 250μg/mL extract. The antimicrobial analysis was determined by the Kirby-Bauer method with some adaptations, we used strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia. Were used three crude extract concentrations. The concentration of 25mg/mL and 50mg/mL showed zones of inhibition against the strain of E. coli. These results demonstrate the potential of this species to source of antibacterial compounds. Therefore, the results shown in this study partially justify its popular use. Keywords: Natural products; soursop; secondary metabolites.