Biosynthesis of Silver Nanoparticles on Orthodontic Elastomeric Modules: Evaluation of Mechanical and Antibacterial Properties
Alma E. Hernández-Gómora,
Edith Lara-Carrillo,
Julio B. Robles-Navarro,
Rogelio J. Scougall-Vilchis,
Susana Hernández-López,
Carlo E. Medina-Solís,
Raúl A. Morales-Luckie
Affiliations
Alma E. Hernández-Gómora
Facultad de Odontología, Universidad Autónoma del Estado de México, Jesús Carranza y Paseo Tollocan, 50120 Toluca, Estado de México, Mexico
Edith Lara-Carrillo
Facultad de Odontología, Universidad Autónoma del Estado de México, Jesús Carranza y Paseo Tollocan, 50120 Toluca, Estado de México, Mexico
Julio B. Robles-Navarro
Facultad de Odontología, Universidad Autónoma del Estado de México, Jesús Carranza y Paseo Tollocan, 50120 Toluca, Estado de México, Mexico
Rogelio J. Scougall-Vilchis
Centro de Investigación y Estudios Avanzados en Odontología, Universidad Autónoma del Estado de México, Jesús Carranza y Paseo Tollocan, 50130 Toluca, Estado de México, Mexico
Susana Hernández-López
Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan S/N, 50120 Toluca, Estado de México, Mexico
Carlo E. Medina-Solís
Área Académica de Odontología, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, 42039 Pachuca, Hidalgo, Mexico
Raúl A. Morales-Luckie
Centro Conjunto de Investigación en Química Sustentable UAEM-UNAM, Universidad Autónoma del Estado de México, Carretera Toluca-Atlacomulco Km 14.5, San Cayetano, 50200 Toluca, Estado de México, Mexico
In the present study, silver nanoparticles (AgNPs) were synthesized in situ on orthodontic elastomeric modules (OEM) using silver nitrate salts as metal-ion precursors and extract of the plant Hetheroteca inuloides (H. inuloides) as bioreductant via a simple and eco-friendly method. The synthesized AgNPs were characterized by UV-visible spectroscopy; scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and transmission electron microscopy (TEM). The surface plasmon resonance peak found at 472 nm confirmed the formation of AgNPs. SEM and TEM images reveal that the particles are quasi-spherical. The EDS analysis of the AgNPs confirmed the presence of elemental silver. The antibacterial properties of OEM with AgNPs were evaluated against the clinical isolates Streptococcus mutans, Lactobacillus casei, Staphylococcus aureus and Escherichia coli using agar diffusion tests. The physical properties were evaluated by a universal testing machine. OEM with AgNPs had shown inhibition halos for all microorganisms in comparison with OEM control. Physical properties increased with respect to the control group. The results suggest the potential of the material to combat dental biofilm and in turn decrease the incidence of demineralization in dental enamel, ensuring their performance in patients with orthodontic treatment.
