Gayana (Jan 2002)
ESTUDIO POBLACIONAL DEL RECURSO ANCHOVETA (ENGRAULIS RINGENS JENYNS 1842) (CLUPEIFORMES, ENGRAULIDAE), MEDIANTE ANALISIS DE ADN POPULATION STUDY OF THE ANCHOVY RESOURCE (ENGRAULIS RINGENS JENYNS 1842) (CLUPEIFORMES, ENGRAULIDAE), THROUGH DNA ANALYSIS
Abstract
Se estudió la variabilidad de 2 genes nucleares, el intrón 4/5 del gen de Calmodulina (CaM) por amplificación PCR y la región ITS (Internal Transcribed Spacers) del ADNr, por PCR y digestión en 139 ejemplares de E. ringens provenientes de las costas de Iquique (20°13'S; 70°09'W) y Talcahuano (36°41'S; 73°06'W). Para CaM se identificaron 8 genotipos en las muestras de Talcahuano y 7 en Iquique, presentando 1 a 3 fragmentos amplificados de 550 a 1.100 pb. En la digestión del fragmento ITS amplificado, sólo la endonucleasa TaqªI detectó 3 diferentes genotipos, 2 presentes en Talcahuano y 3 en Iquique. Se discuten las frecuencias observadas para cada gen y área de estudio. Los resultados llevan a confirmar la existencia de un solo stock, desde un punto de vista genéticoThe genetic variability of two nuclear genes, the 4/5 intron of the Calmodulin (CaM) and the ITS (Internal Transcribed Spacers) of the DNAr were studied. The methodology applied in 139 anchovies (E. ringens) from Iquique and Talcahuano, were the PCR amplification for CaM samples and the PCR amplification and digestion, for ITS samples. The results exhibited 8 genotypes for CaM in Talcahuano, and 7 were identified in Iquique. The size of the fragments founded (1-3), ranged from 550 to 1100 bp. The ITS amplification and digestion of a fragment determined the existence of 3 genotypes, 2 in Talcahuano and 3 in Iquique. But in the digestion of the ITS amplifications, only the TaqªI endonuclease showed diferences in the restriction profiles. The genotypic frequencies are discussed for each gene and study area, as well as the presence of only one stock from a genetic point of view
