Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor

Erika Cosset; Tieng Vannary; Frédérique Sloan-Béna; Stefania Gimelli; Eric Gerstel; Karl-Heinz Krause; Antoine Marteyn

Stem Cell Research (Oct 2020)

Generation of human induced pluripotent stem cell line UNIGEi003-A from skin fibroblasts of an apparently healthy male donor

Erika Cosset,
Tieng Vannary,
Frédérique Sloan-Béna,
Stefania Gimelli,
Eric Gerstel,
Karl-Heinz Krause,
Antoine Marteyn

Affiliations

Erika Cosset: Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Tieng Vannary: Vanarix SA, Lausanne, Switzerland
Frédérique Sloan-Béna: Service of Genetic Medicine, Geneva University Hospitals, Geneva, Switzerland
Stefania Gimelli: Service of Genetic Medicine, Geneva University Hospitals, Geneva, Switzerland
Eric Gerstel: Hirslanden Clinique La Colline, Geneva, Switzerland; Department of Internal Medicine, Department of Primary Care Medicine, Clinical Epidemiology Division, Department of Community Health and Medicine, Geneva University Hospital, Geneva, Switzerland
Karl-Heinz Krause: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Antoine Marteyn: Laboratory of Tumor Immunology, Translational Research Center in Onco-Hematology, Department of Internal Medicine Specialties, Faculty of Medicine, University of Geneva, Geneva, Switzerland; Corresponding author.

Journal volume & issue: Vol. 48
p. 101928

Abstract

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Dermal fibroblasts isolated from an apparently healthy 50-year-old man were successfully transformed into induced pluripotent stem cells (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The generated iPSC line has been expanded under feeder-free conditions and displayed all hallmarks of a standard pluripotent stem cell line such as a normal karyotype, expression of pluripotent factors and differentiation capacity into the three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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