Aspergillus niger ATCCC 11414 in a media with Anacardium occidentale gum showed the morphological characteristics (macro- and microscopic) that led its identification. It was studied many growth conditions in comparison with Czapeck Dox Agar. The best growth of the fungus, observed at given conditions (pH 4.5; 6.2 , presence of magnesium ions), was chosen to study the interaction fungus-gum which was demonstrated by the enzymatical activity. The production of -D-galactosidase, supports by the presence of -D-Galp-(16)- Galp in the branches of A. occidentale gum structure, may suggest that galactose is used as carbon source by the fungus. The results showed that the media with A. occidentale gum is a good one for A.niger growth and may be useful for biotechnology application.