Cancer Management and Research (Jul 2021)
LncRNA NEAT1 Acts as an miR-148b-3p Sponge to Regulate ROCK1 Inhibition of Retinoblastoma Growth
Abstract
Hang Lu,1,* Zhenjun Zhang,2,* Yao Lu,3 Weiwei Xiu,1 Jinglin Cui1 1Research Center of Ophthalmology, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, Qiqihar, Heilongjiang Province, People’s Republic of China; 2Ophthalmology Department, Beiman Hongpeng Hospital of Qiqihar, Qiqihar, Heilongjiang Province, People’s Republic of China; 3International Education College, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinglin CuiResearch Center of Ophthalmology, The First Hospital of Qiqihar, Affiliated Qiqihar Hospital, Southern Medical University, No. 30 Gongyuan Road, Longsha District, Qiqihar, Heilongjiang Province, People’s Republic of ChinaEmail [email protected]: It is reported that long non-coding RNA nuclear paraspeckle assembly transcript 1 (LncRNA NEAT1) is involved in the occurrence and development of various cancers. However, the detailed biological function and mechanism of LncRNA NEAT1 in retinoblastoma are still unclear. So we will explore the biological function and possible mechanism of LncRNA NEAT1 in retinoblastoma.Materials and Methods: Quantitative real-time PCR (qRT-PCR) was used to detect LncRNA NEAT1 in retinoblastoma tissues and cell lines. Cell counting kit 8, Transwell and flow cytometry were applied to explore cell proliferation, invasion and apoptosis. The target miRNAs (miR) of LncRNA NEAT1 and miR and downstream target genes were predicted using Starbase3.0 software and confirmed by double luciferase reporting test and RNA binding protein immunoprecipitation (RIP). Western Blot was applied to explore ROCK1 in cells, and tumor allogeneic experiment was applied to study the role of LncRNA NEAT1 on tumor growth.Results: It was found that LncRNA NEAT1 was up-regulated in retinoblastoma tissues, cells and serum, and the prognosis of patients with high expression of LNC RNA NEAT 1 was poor. Functional analysis showed that knocking down LncRNA NEAT1 could weaken proliferation and invasion, and accelerate apoptosis. Tumor allogeneic experiment showed that sh-NEAT1 injection can inhibit tumor growth. In addition, LncRNA NEAT1 inhibited proliferation and invasion, and promoted apoptosis through miR-148b-3p/ROCK1 axis.Conclusion: LncRNA NEAT1 can mediate miR-148b-3p/ROCK1 axis to weaken the proliferation and invasion of retinoblastoma.Keywords: LncRNA NEAT1, miR-148b-3p, ROCK1, retinoblastoma, proliferation, invasion
