Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase

Molecules. 2011;16(6):4807-4817 DOI 10.3390/molecules16064807

 

Journal Homepage

Journal Title: Molecules

ISSN: 1420-3049 (Print)

Publisher: MDPI AG

LCC Subject Category: Science: Chemistry: Organic chemistry

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Ana Lúcia Figueiredo Porto
Keila Aparecida Moreira
Maria Luiza Carvalho Neves
Tatiana Souza Porto
Carlos Ricardo Soccol
Michele Rigon Spier
Cristina Maria Souza-Motta
Milena Fernandes da Silva

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 11 weeks

 

Abstract | Full Text

Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 23 factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.