Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase

Molecules. 2011;16(6):4807-4817 DOI 10.3390/molecules16064807


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Journal Title: Molecules

ISSN: 1420-3049 (Print)

Publisher: MDPI AG

LCC Subject Category: Science: Chemistry: Organic chemistry

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML



Ana Lúcia Figueiredo Porto

Keila Aparecida Moreira

Maria Luiza Carvalho Neves

Tatiana Souza Porto

Carlos Ricardo Soccol

Michele Rigon Spier

Cristina Maria Souza-Motta

Milena Fernandes da Silva


Blind peer review

Editorial Board

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Time From Submission to Publication: 11 weeks


Abstract | Full Text

Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 23 factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.