Efficacy of native entomopathogenic fungus, Isaria fumosorosea, against bark and ambrosia beetles, Anisandrus dispar Fabricius and Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Abstract

Abstract The efficacy of the native entomopathogenic fungus, Isaria fumosorosea TR-78-3, was evaluated against females of the bark and ambrosia beetles, Anisandrus dispar Fabricius and Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae), under laboratory conditions by two different methods as direct and indirect treatments. In the first method, conidial suspensions (1 × 106 and 1 × 108 conidia ml−1) of the fungus were directly applied to the beetles in Petri dishes (2 ml per dish), using a Potter spray tower. In the second method, the same conidial suspensions were applied on a sterile hazelnut branch placed in the Petri dishes. The LT50 and LT90 values of 1 × 108 conidia ml−1 were 4.78 and 5.94/days, for A. dispar in the direct application method, while they were 4.76 and 6.49/days in the branch application method. Similarly, LT50 and LT90 values of 1 × 108 conidia ml−1 for X. germanus were 4.18 and 5.62/days, and 5.11 and 7.89/days, for the direct and branch application methods, respectively. The efficiency of 1 × 106 conidia ml− 1 was lower than that of 1 × 108 against the beetles in both application methods. This study indicates that I. fumosorosea TR-78-3 had a significant potential as a biological control agent against A. dispar and X. germanus. Further studies are necessary to evaluate the efficacy of the isolate on the pests under field conditions.

Keywords

• Bark and ambrosia beetles
• Biological control
• Entomopathogenic fungus
• Isaria fumosorosea
• Hazelnut