Bacillus velezensis GX1 and its potential for the control of lily bulb rot

Sha Zhang; Ting Lou; Si Wu; Shenggui Liu; Hao Pan; Jing Yang; Yifeng Su; Wang Hu; Wei Xiang; Shuangyan Pan; Lei Sun

Biological Control (Nov 2024)

Bacillus velezensis GX1 and its potential for the control of lily bulb rot

Sha Zhang,
Ting Lou,
Si Wu,
Shenggui Liu,
Hao Pan,
Jing Yang,
Yifeng Su,
Wang Hu,
Wei Xiang,
Shuangyan Pan,
Lei Sun

Affiliations

Sha Zhang: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Ting Lou: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Si Wu: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Shenggui Liu: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Hao Pan: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Jing Yang: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Yifeng Su: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Wang Hu: State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China
Wei Xiang: College of Horticulture, Hunan Agricultural University, Changsha 410128, China
Shuangyan Pan: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China
Lei Sun: Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China; Corresponding author.

Journal volume & issue: Vol. 198
p. 105616

Abstract

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Bulb rot of lily (Lilium brownii var. viridulum), caused by Fusarium commune, is a serious soilborne disease of lily plants. In this study, several bacterial isolates were obtained from the rhizosphere of healthy lily plants and evaluated for biocontrol potential. Among the obtained isolates, GX1 had the highest level of antifungal activity in vitro, inhibiting the growth of F. commune by 52.0 %, relative to the control (CK). The isolate was identified as Bacillus velezensis based on its morphology and 16S rRNA and gyrB gene sequence analysis. The fermentation broth of GX1 after 12 d of fermentation at 25 °C (pH 7) exhibited strong antifungal activity. A crude lipopeptide extracts of GX1 fermentation broth was as effective (50.3 %) at inhibiting the mycelial growth of F. commune as living cultures of GX1. GX1 possesses fenD, bmyB, srfAA and bacA antimicrobial peptide (AMP) genes, which may be the primary source of its antifungal activity. GX1 significantly reduced the disease severity index of lily bulb rot in pot experiments, indicating that B. velezensis GX1 has substantial potential for the biological control of lily bulb rot.

Published in Biological Control

ISSN: 1049-9644 (Print); 1090-2112 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture; Science: Biology (General)
Website: https://www.sciencedirect.com/journal/biological-control

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