Nature Communications (Oct 2019)
A reference map of murine cardiac transcription factor chromatin occupancy identifies dynamic and conserved enhancers
- Brynn N. Akerberg,
- Fei Gu,
- Nathan J. VanDusen,
- Xiaoran Zhang,
- Rui Dong,
- Kai Li,
- Bing Zhang,
- Bin Zhou,
- Isha Sethi,
- Qing Ma,
- Lauren Wasson,
- Tong Wen,
- Jinhua Liu,
- Kunzhe Dong,
- Frank L. Conlon,
- Jiliang Zhou,
- Guo-Cheng Yuan,
- Pingzhu Zhou,
- William T. Pu
Affiliations
- Brynn N. Akerberg
- Department of Cardiology, Boston Children’s Hospital
- Fei Gu
- Department of Cardiology, Boston Children’s Hospital
- Nathan J. VanDusen
- Department of Cardiology, Boston Children’s Hospital
- Xiaoran Zhang
- Department of Cardiology, Boston Children’s Hospital
- Rui Dong
- Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute
- Kai Li
- Department of Cardiology, Boston Children’s Hospital
- Bing Zhang
- Xin Hua Hospital, Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
- Bin Zhou
- Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences
- Isha Sethi
- Department of Cardiology, Boston Children’s Hospital
- Qing Ma
- Department of Cardiology, Boston Children’s Hospital
- Lauren Wasson
- Biology Department, University of North Carolina at Chapel Hill
- Tong Wen
- Department of Cardiology, The First Affiliated Hospital of Nanchang University
- Jinhua Liu
- Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University
- Kunzhe Dong
- Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University
- Frank L. Conlon
- Biology Department, University of North Carolina at Chapel Hill
- Jiliang Zhou
- Department of Pharmacology & Toxicology, Medical College of Georgia, Augusta University
- Guo-Cheng Yuan
- Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute
- Pingzhu Zhou
- Department of Cardiology, Boston Children’s Hospital
- William T. Pu
- Department of Cardiology, Boston Children’s Hospital
- DOI
- https://doi.org/10.1038/s41467-019-12812-3
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 16
Abstract
Mapping transcription factors (TFs) occupancy is essential for understanding transcriptional programs. Here the authors use biotinylated knockin alleles of key cardiac TFs (GATA4, NKX2-5, MEF2A, MEF2C, SRF, TBX5, TEAD1) to map their genome-wide occupancy in the fetal and adult mouse heart, providing insight into the cardiac transcriptional regulatory network.
