Nature Communications (Oct 2019)

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

  • William H. Gittens,
  • Dominic J. Johnson,
  • Rachal M. Allison,
  • Tim J. Cooper,
  • Holly Thomas,
  • Matthew J. Neale

DOI
https://doi.org/10.1038/s41467-019-12802-5
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 16

Abstract

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Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs). Here the authors develop CC-seq—a method that allows direct mapping of both Top2-linked SSBs and DSBs—and reveal a significant impact of primary DNA sequence on Top2 directed cleavage.