FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS

Abstract

The aim of the work was to study the parameters of T-cells, humoral immunity and activity of NAD(P)- dependent dehydrogenases in lymphocytes from peripheral blood at young children with recurrent obstructive bronchitis (ROB). Patients and Methods: Fifty-four children at the age of 1-3 years with ROB were observed. The control group consisted of 35 healthy children at similar age. The numbers of СD3+, СD4+, СD8+, СD19+ cells in peripheral blood were determined by flow cytofluorimetric technique. IgA, IgM, and IgG concentrations in blood serum were evaluated by G. Mancini et al. (1965); circulating immune complexes (CIC), by V. Haskova et al. (1978); total IgE test was performed with “Total IgE – IFA Best” system (Russia). Relative activity of NAD (P)-dependent dehydrogenase in peripheral blood lymphocytes was studied according to A. Savchenko et al.(1989). The results of this study have revealed changed phenotypic spectrum of peripheral blood lymphocyte populations; a statistically significant reduction in IgA concentration, and a tendency for increased IgE levels in blood serum in the children with ROB. An increase of ribose-5-phosphate- and NADPdependent metabolic processes; reduced lactate dehydrogenase anaerobic reactions; a switch from lipid catabolism products to glycolysis; a shunt role of malate/aspartate in cell energy supply, and for glutathione reductase activity; elevated levels of substrate flow along Krebs cycle in peripheral blood lymphocytes were registered as well. It may be concluded that the change of phenotype and enzymatic activity of blood lymphocytes, selective IgA deficiency in blood serum could be shown in the younger children with recurrent obstructive bronchitis.

Keywords

• bronchial obstruction
• bronchitis
• recurrent
• lymphocyte
• phenotype
• immunoglobulins
• metabolism