Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease (Jan 2016)

Histopathological Correlates of Global and Segmental Left Ventricular Systolic Dysfunction in Experimental Chronic Chagas Cardiomyopathy

  • Luciano Fonseca Lemos de Oliveira,
  • Minna Moreira Dias Romano,
  • Eduardo Elias Vieira de Carvalho,
  • Jorge Mejia Cabeza,
  • Hélio Cesar Salgado,
  • Rubens Fazan Júnior,
  • Renata Sesti Costa,
  • João Santana da Silva,
  • Maria de Lourdes Higuchi,
  • Benedito Carlos Maciel,
  • Edécio Cunha‐Neto,
  • José Antônio Marin‐Neto,
  • Marcus Vinícius Simões

DOI
https://doi.org/10.1161/JAHA.115.002786
Journal volume & issue
Vol. 5, no. 1

Abstract

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BackgroundChronic Chagas cardiomyopathy in humans is characterized by segmental left ventricular wall motion abnormalities (WMA), mainly in the early stages of disease. This study aimed at investigating the detection of WMA and its correlation with the underlying histopathological changes in a chronic Chagas cardiomyopathy model in hamsters. Methods and ResultsFemale Syrian hamsters (n=34) infected with 3.5×104 or 105 blood trypomastigote Trypanosoma cruzi (Y strain) forms and an uninfected control group (n=7) were investigated. After 6 or 10 months after the infection, the animals were submitted to in vivo evaluation of global and segmental left ventricular systolic function by echocardiography, followed by euthanasia and histological analysis for quantitative assessment of fibrosis and inflammation with tissue sampling in locations coinciding with the left ventricular wall segmentation employed at the in vivo echocardiographic evaluation. Ten of the 34 infected animals (29%) showed reduced left ventricular ejection fraction (<73%). Left ventricular ejection fraction was more negatively correlated with the intensity of inflammation (r=−0.63; P<0.0001) than with the extent of fibrosis (r=−0.36; P=0.036). Among the 24 animals with preserved left ventricular ejection fraction (82.9±5.5%), 8 (33%) showed segmental WMA predominating in the apical, inferior, and posterolateral segments. The segments exhibiting WMA, in comparison to those with normal wall motion, showed a greater extent of fibrosis (9.3±5.7% and 7±6.3%, P<0.0001) and an even greater intensity of inflammation (218.0±111.6 and 124.5±84.8 nuclei/mm², P<0.0001). ConclusionsIsolated WMA with preserved global systolic left ventricular function is frequently found in Syrian hamsters with experimental chronic Chagas cardiomyopathy whose underlying histopathological features are mainly inflammatory.

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