Glycolytic enzyme inhibitory and antiglycation potential of rutin

Abstract

The aim of the present was to determine the mechanism for the antidiabetic potential exerted by rutin by studying its effect on the glycolytic enzyme inhibitory and antiglycation potential. For the determination of glycolytic enzyme inhibitory potential of rutin, porcine pancreatic amylase inhibitory assay and α-glucosidase inhibitory assay were performed. The antiglycation potential was determined by glycation of bovine serum albumin followed by antiglycation estimation of fructosamines adducts, protein carbonyls, protein thiols, congo red absorbance. Rutin showed a significant inhibition of α-amylase (p < 0.001; 53.66%) and α-glucosidase (p < 0.001; 52.56%). To study antiglycation potential, various parameters were determined and fructosamine inhibition was found to be 35.55%, protein carbonyls were inhibited up to 13.49%. Protein thiols were inhibited up to 80.27%. In the present study, it was concluded that rutin showed glycolytic enzyme inhibitory and antiglycation potential.

Keywords

• α-amylase
• α-glucosidase
• Antiglycation
• Docking
• Rutin