Characterization and In Vitro and In Vivo Assessment of a Novel Cellulose Acetate-Coated Mg-Based Alloy for Orthopedic Applications
Patricia Neacsu,
Adela Ioana Staras,
Stefan Ioan Voicu,
Iuliana Ionascu,
Teodoru Soare,
Seralp Uzun,
Vasile Danut Cojocaru,
Andreea Madalina Pandele,
Sorin Mihai Croitoru,
Florin Miculescu,
Cosmin Mihai Cotrut,
Ioan Dan,
Anisoara Cimpean
Affiliations
Patricia Neacsu
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Spl. Independentei, 050095 Bucharest, Romania
Adela Ioana Staras
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Spl. Independentei, 050095 Bucharest, Romania
Stefan Ioan Voicu
Department of Analytical Chemistry and Environmental Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Iuliana Ionascu
Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine, 105 Spl. Independentei, 050097 Bucharest, Romania
Teodoru Soare
Pathology Department, University of Agricultural Sciences and Veterinary Medicine, 105 Spl. Independentei, 050097 Bucharest, Romania
Seralp Uzun
Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine, 105 Spl. Independentei, 050097 Bucharest, Romania
Vasile Danut Cojocaru
Materials Processing Department, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Andreea Madalina Pandele
Department of Analytical Chemistry and Environmental Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Sorin Mihai Croitoru
Machines and Manufacturing Systems Department, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Florin Miculescu
Department of Metallic Materials Science, Physical Metallurgy, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Cosmin Mihai Cotrut
Department of Metallic Materials Science, Physical Metallurgy, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania
Ioan Dan
SC R&D Consulting and Services SRL, 45 Maria Ghiculeasa, 023761 Bucharest, Romania
Anisoara Cimpean
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Spl. Independentei, 050095 Bucharest, Romania
Despite their good biocompatibility and adequate mechanical behavior, the main limitation of Mg alloys might be their high degradation rates in a physiological environment. In this study, a novel Mg-based alloy exhibiting an elastic modulus E = 42 GPa, Mg-1Ca-0.2Mn-0.6Zr, was synthesized and thermo-mechanically processed. In order to improve its performance as a temporary bone implant, a coating based on cellulose acetate (CA) was realized by using the dipping method. The formation of the polymer coating was demonstrated by FT-IR, XPS, SEM and corrosion behavior comparative analyses of both uncoated and CA-coated alloys. The potentiodynamic polarization test revealed that the CA coating significantly improved the corrosion resistance of the Mg alloy. Using a series of in vitro and in vivo experiments, the biocompatibility of both groups of biomaterials was assessed. In vitro experiments demonstrated that the media containing their extracts showed good cytocompatibility on MC3T3-E1 pre-osteoblasts in terms of cell adhesion and spreading, viability, proliferation and osteogenic differentiation. In vivo studies conducted in rats revealed that the intramedullary coated implant for fixation of femur fracture was more efficient in inducing bone regeneration than the uncoated one. In this manner, the present study suggests that the CA-coated Mg-based alloy holds promise for orthopedic aplications.
