Natural Products Isolated from Oriental Medicinal Herbs Inactivate Zika Virus
Mariana N. Batista,
Ana Cláudia S. Braga,
Guilherme Rodrigues Fernandes Campos,
Marcos Michel Souza,
Renata Prandini Adum de Matos,
Tairine Zara Lopes,
Natalia Maria Candido,
Maria Leticia Duarte Lima,
Francielly Cristina Machado,
Stephane Tereza Queiroz de Andrade,
Cíntia Bittar,
Maurício L. Nogueira,
Bruno M. Carneiro,
Ricardo B. Mariutti,
Raghuvir Krishnaswamy Arni,
Marilia Freitas Calmon,
Paula Rahal
Affiliations
Mariana N. Batista
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Ana Cláudia S. Braga
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Guilherme Rodrigues Fernandes Campos
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Marcos Michel Souza
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Renata Prandini Adum de Matos
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Tairine Zara Lopes
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Natalia Maria Candido
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Maria Leticia Duarte Lima
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Francielly Cristina Machado
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Stephane Tereza Queiroz de Andrade
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Cíntia Bittar
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Maurício L. Nogueira
Departamento de Doenças Infecciosas e Parasitárias, FAMERP, São José do Rio Preto 15090-000, São Paulo, Brazil
Bruno M. Carneiro
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Ricardo B. Mariutti
Department of Physics, Multiuser Center for Biomolecular Innovation, Sao Paulo State University-UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil;<email>[email protected]</email> (R.B.M.)
Raghuvir Krishnaswamy Arni
Department of Physics, Multiuser Center for Biomolecular Innovation, Sao Paulo State University-UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil;<email>[email protected]</email> (R.B.M.)
Marilia Freitas Calmon
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Paula Rahal
Laboratory of Genomic Studies, Sao Paulo State University—UNESP, São José do Rio Preto 15054-000, São Paulo, Brazil
Zika virus (ZIKV) has been associated with serious health conditions, and an intense search to discover different ways to prevent and treat ZIKV infection is underway. Berberine and emodin possess several pharmacological properties and have been shown to be particularly effective against the entry and replication of several viruses. We show that emodin and berberine trigger a virucidal effect on ZIKV. When the virus was exposed to 160 µM of berberine, a reduction of 77.6% in the infectivity was observed; when emodin was used (40 µM), this reduction was approximately 83.3%. Dynamic light scattering data showed that both compounds significantly reduce the hydrodynamic radius of virus particle in solution. We report here that berberine and emodin, two natural compounds, have strong virucidal effect in Zika virus.
