Synthesis and characterization of 1- and 2-monoglycerides of anteiso fatty acids

Abstract

The branched-chain fatty acids d-(+)-12-methyltetradecanoic acid (C15 anteiso) and d-(+)-14-methylhexadecanoic acid (C17, anteiso) were isolated from the lipids of Listeria monocytogenes and their 1- and 2-monoglycerides were prepared. Reaction intermediates and products were purified without isomerization by column chromatography. Thin-layer chromatography on Florisil impregnated with boric acid and nuclear magnetic resonance were used in characterizing the 1- and 2-monoglycerides. The value of the latter method for analyzing glyceride structure is discussed.

Keywords

• bacterial lipids
• anteiso fatty acids
• 1- and 2-monoglycerides
• synthesis
• chromatography
• column