Betulin-3,28-diphosphate as a Component of Combination Cytostatic Drugs for the Treatment of Ehrlich Ascites Carcinoma In Vitro and In Vivo Experiments

Olga Vorobyova; Olga Deryabina; Darina Malygina; Nadezhda Plotnikova; Anna Solovyeva; Kseniya Belyaeva; Nina Melnikova

doi:10.3390/scipharm86020017

Scientia Pharmaceutica (Apr 2018)

Betulin-3,28-diphosphate as a Component of Combination Cytostatic Drugs for the Treatment of Ehrlich Ascites Carcinoma In Vitro and In Vivo Experiments

Olga Vorobyova,
Olga Deryabina,
Darina Malygina,
Nadezhda Plotnikova,
Anna Solovyeva,
Kseniya Belyaeva,
Nina Melnikova

Affiliations

Olga Vorobyova: Department of Pharmaceutical Chemistry, Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University”, Ministry of Health of the Russian Federation, Minin sq., 10/1, 603005 Nizhny Novgorod, Russia
Olga Deryabina: Department of Chemistry, N.P. Ogarev Mordovian State University, Bolshevistskaya St. 68, 430005 Saransk, Russia
Darina Malygina: Department of Pharmaceutical Chemistry, Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University”, Ministry of Health of the Russian Federation, Minin sq., 10/1, 603005 Nizhny Novgorod, Russia
Nadezhda Plotnikova: Department of Chemistry, N.P. Ogarev Mordovian State University, Bolshevistskaya St. 68, 430005 Saransk, Russia
Anna Solovyeva: Department of Pharmaceutical Chemistry, Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University”, Ministry of Health of the Russian Federation, Minin sq., 10/1, 603005 Nizhny Novgorod, Russia
Kseniya Belyaeva: Department of Pharmaceutical Chemistry, Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University”, Ministry of Health of the Russian Federation, Minin sq., 10/1, 603005 Nizhny Novgorod, Russia
Nina Melnikova: Department of Pharmaceutical Chemistry, Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University”, Ministry of Health of the Russian Federation, Minin sq., 10/1, 603005 Nizhny Novgorod, Russia

DOI: https://doi.org/10.3390/scipharm86020017
Journal volume & issue: Vol. 86, no. 2
p. 17

Abstract

Read online

The activity of betulin-3,28-diphosphate (BDP) in combination with the cytostatics such as 5-fluorouracil (5-FU) and hydrazine sulfate (HS) was demonstrated by using the transplanted Ehrlich ascites carcinoma (EAC) in mice. The dose-dependent effect of combination drugs BDP + HS and BDP + 5-FU was revealed by in vitro experiments on rats. The synergetic effect of HS and BDP on oxidative stress and energy metabolism was established. The malonic dialdehyde (MDA) level both in plasma and erythrocytes decreased by 87 ± 2%, and the superoxide dismutase (SOD) activity increased by 105 ± 7% in comparison with the control. The combination of BDP + HS promoted the increase of lactate dehydrogenase (LDH) activity in the reverse reaction by 195 ± 21% compared to the control. The combination drug of 5-FU with BDP caused the synergetic decrease of the lipid peroxidation (LPO) intensity estimated by the MDA level decrease up to 14 ± 4% compared to pure compounds. Betulin-3,28-diphosphate in combination with cytostatics for EAC treatment improved the animal health status, as well as decreased the cytostatics dose that can be used in palliative therapy.

Published in Scientia Pharmaceutica

ISSN: 0036-8709 (Print); 2218-0532 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/scipharm

About the journal

Abstract

Keywords