Cancer Management and Research (Nov 2020)

Silencing circSLC19A1 Inhibits Prostate Cancer Cell Proliferation, Migration and Invasion Through Regulating miR-326/MAPK1 Axis

  • Huang B,
  • Zhou D,
  • Huang X,
  • Xu X,
  • Xu Z

Journal volume & issue
Vol. Volume 12
pp. 11883 – 11895

Abstract

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Banggao Huang,1 Danhong Zhou,2 Xinmian Huang,1 Xiaobo Xu,1 Zhihui Xu1 1Department of Urology, Zhejiang Provincial People’s Hospital, Hangzhou, Zhejiang Province, People’s Republic of China; 2Department of Surgery, The Third People’s Hospital of Hangzhou, Hangzhou, Zhejiang Province, People’s Republic of ChinaCorrespondence: Zhihui XuDepartment of Urology, Zhejiang Provincial People’s Hospital, No. 158, Shangtang Road, Hangzhou 310014, People’s Republic of ChinaTel +86-571-87666666Fax +86-571-85335800Email [email protected]: Emerging evidence indicates that circular RNAs (circRNAs), which form as covalently closed loops, play a regulatory role in various types of cancer, including prostate cancer (PCa). CircSLC19A1, one kind of circRNA, was subjected to the study and its role in PCa was explored.Methods: Expressions of circSLC19A1, miR-326 and MAPK1 in PCa tissues and cells were assessed by qRT-PCR. CircSLC19A1 was identified by RNase R treatment. The binding relations between circSLC19A1 and miR-326 and between miR-326 and MAPK1 were predicted by RegRNA2.0 or Targetscan7.2 and further confirmed by dual-luciferase reporter assay. Pearson correlation analysis of the correlation among circSLC19A1, miR-326 and MAPK1 was performed. CCK-8, cell colony formation, wound healing and Transwell assays were used to assess PCa cell viability, proliferation, migration and invasion, respectively.Results: CircSLC19A1 expression was up-regulated in PCa tissue and cell cytoplasm. Silencing circSLC19A1 inhibited PCa cell viability, proliferation, migration, invasion and miR-326 expression. MiR-326 inhibitor promoted the luciferase activities of circSLC19A1 and MAPK1, increased MAPK1 expression and facilitated PCa cell progression. MiR-326 expression was down-regulated in PCa tissue and there was a negative correlation between miR-326 and circSLC19A1 expressions. MAPK1 expression was up-regulated in PCa tissue. There was a negative correlation between MAPK1 and miR-326 expressions as well as a positive correlation between MAPK1 and circSLC19A1 expressions. Silencing MAPK1 promoted the viability, proliferation, migration, and invasion of PCa cells co-transfected with siRNA-circSLC19A1a and miR-326 inhibitor.Conclusion: CircSLC19A1 silencing inhibited PCa cell proliferation, migration and invasion through regulating miR-326/MAPK1 axis.Keywords: circSLC19A1, miR-326, MAPK1, prostate cancer, proliferation, migration, invasion

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