Nature Communications (Jun 2019)

Addendum: Precise tuning of gene expression levels in mammalian cells

  • Yale S. Michaels,
  • Mike B. Barnkob,
  • Hector Barbosa,
  • Toni A. Baeumler,
  • Mary K. Thompson,
  • Violaine Andre,
  • Huw Colin-York,
  • Marco Fritzsche,
  • Uzi Gileadi,
  • Hilary M. Sheppard,
  • David J. H. F. Knapp,
  • Thomas A. Milne,
  • Vincenzo Cerundolo,
  • Tudor A. Fulga

DOI
https://doi.org/10.1038/s41467-019-10615-0
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 3

Abstract

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Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.