Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

Yuefeng Li; Kiran Nakka; Thomas Olender; Philippe Gingras-Gelinas; Matthew Man-Kin Wong; Daniel C.L. Robinson; Hina Bandukwala; Carmen G. Palii; Odile Neyret; Marjorie Brand; Alexandre Blais; F. Jeffrey Dilworth

STAR Protocols (Sep 2021)

Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

Yuefeng Li,
Kiran Nakka,
Thomas Olender,
Philippe Gingras-Gelinas,
Matthew Man-Kin Wong,
Daniel C.L. Robinson,
Hina Bandukwala,
Carmen G. Palii,
Odile Neyret,
Marjorie Brand,
Alexandre Blais,
F. Jeffrey Dilworth

Affiliations

Yuefeng Li: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; Corresponding author
Kiran Nakka: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; Corresponding author
Thomas Olender: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
Philippe Gingras-Gelinas: Molecular Biology Platform, Institut de Recherche Clinique de Montreal, Montreal, QC, Canada
Matthew Man-Kin Wong: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
Daniel C.L. Robinson: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
Hina Bandukwala: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada
Carmen G. Palii: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada
Odile Neyret: Molecular Biology Platform, Institut de Recherche Clinique de Montreal, Montreal, QC, Canada
Marjorie Brand: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; LIFE Research Institute, University of Ottawa, Ottawa, ON, Canada
Alexandre Blais: Ottawa Institute for Systems Biology, University of Ottawa, Ottawa, ON, Canada; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada; CI3, University of Ottawa Centre for Infection, Immunity and Inflammation, Ottawa, ON, Canada
F. Jeffrey Dilworth: Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada; LIFE Research Institute, University of Ottawa, Ottawa, ON, Canada; Ottawa Institute for Systems Biology, University of Ottawa, Ottawa, ON, Canada; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100751

Abstract

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Summary: Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34+ hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population.For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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