Di-san junyi daxue xuebao (Oct 2021)
Angelica sinensis polysaccharide antagonizes D-galactose induced brain aging in rats
Abstract
Objective To explore the antagonistic effect of Angelica sinensis polysaccharide (ASP) on brain aging induced by D-galactose (D-gal) in rats. Methods SD rats were randomly divided into control group, ASP group, aging model group, and ASP aging model group. Their spatial learning and memory abilities were detected by water maze test. The aging of brain tissue cells were detectded by aging-related β-galactosidase (SA-β-Gal) staining. The contents of SOD, MDA and GSH in hippocampal homogenate were measured by chromatography, and those of IL-1β and IL-6 in hippocampal homogenate were detected by ELISA. Southern blotting and TRAP-PCR were used to detect telomere length and telomerase activity in the hippocampal cells. Neural stem cells (NSCs) were isolated, cultured and identified from the brain tissue of SD fetal rats. The third-generation NSCs were divided into control group, ASP group, aging model group, and ASP aging model group. The content of MDA in the cells was determined by chromatography. The intracellular ROS level was determined by flow cytometry. qRT-PCR was used to detect the mRNA expression of p19, p21 and p53 of senescence related genes. Results Compared with the aging model group, the injection of ASP significantly improved the spatial learning and memory abilities of aging rats. The number of SA-β-Gal positive cells in the brain tissue was decreased (P < 0.05). The contents of SOD and GSH in the hippocampus were increased, but that of MDA was decreased (P < 0.05). The secretion levels of IL-1β and IL-6 were decreased (P < 0.05). The telomere length and telomerase activity were increased (P < 0.05). NSCs were successfully isolated and identified. Compared with the aging group, the MDA content and ROS level were significantly reduced in the ASP aging group (P < 0.05), and the mRNA levels of senescence-related genes p19, p21, and p53 were decreased (P < 0.05). Conclusion ASP may regulate cell telomere length and telomerase activity, down-regulate the expression of aging-related genes, reduce the expression of inflammatory factors, improve the cell's antioxidant capacity, and then antagonize the degeneration effect of D-gal on NSCs and hippocampal cells.
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