Mìkrobìologìâ ì Bìotehnologìâ (Mar 2015)
EFFECT OF LOW-INTENSITY LASER IRRADIATION ON THE CULTIVATED MACROMYCETES SEED CULTURE GROWTH ACTIVITY
Abstract
Aim. The study of the effect of low-intensity laser radiation on the growth processes of spores and mycelia of cultured macromycetes. Methods. The research objects were pure cultures of basidiomycetes Flammulina velutipes 1974, Ganoderma applanatum, Ganoderma lucidum 1887, Hericium erinaceus 963, Lentinus edodes 520 и Pleurotus ostreatus 531. Helium-neon laser with radiation at the wavelength of 632.8 nm and argon ion laser 514.5 nm and 488.0 nm were used as the sources of coherent light. Irradiation has been carried out at the stages of spore germination and development of the vegetative mycelium. Results. The significant differences in photosensitivity between the species at different stages of ontogenesis have been revealed. The analysis of growth parameters allowed us to determine the optimal pacing modes for studied strains. It is expedient to carry out the light processing of F. velutipes, G. applanatum and P. ostreatus seed mycelium in the exponential and stationary growth phases, while for G. lucidum it should be done in the stationary phase. Irradiation of H. erinaceus spores by laser light with the wavelength 488 nm leads to the maximum stimulation of growth processes and biomass accumulation increased almost three times compared with the control. The photostimulation by red and blue light is equally effective at all the examined stages of L. edodes ontogenesis. The use of activated inoculum has reduced four times the number of its entry in the substrate. It has been established that the vegetative mycelium, which was formed from spores modified under the light influence, retains its ability to accelerate the growth and changes induced by light can be transmitted to the further ontogenetic stage from spores to mycelium. Conclusions. Low-intensity laser radiation can be effectively used to increase the biological activity of seed culture cultivated macromycetes to stimulate their growth and intensification of technological stages of cultivation.
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