Arhiv za Higijenu Rada i Toksikologiju (Sep 2022)

Redox and biometal status in Wistar rats after subacute exposure to fluoride and selenium counter-effects

  • Radovanović Jelena,
  • Antonijević Biljana,
  • Baralić Katarina,
  • Ćurčić Marijana,
  • Đukić-Ćosić Danijela,
  • Bulat Zorica,
  • Javorac Dragana,
  • Buha Đorđević Aleksandra,
  • Kotur-Stevuljević Jelena,
  • Sudar-Milovanović Emina,
  • Antonijević Miljaković Evica,
  • Beloica Miloš,
  • Mandinić Zoran

DOI
https://doi.org/10.2478/aiht-2022-73-3650
Journal volume & issue
Vol. 73, no. 3
pp. 207 – 222

Abstract

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This study aimed to investigate the effect of 150 mg/L sodium fluoride (NaF) on redox status parameters and essential metals [copper (Cu), iron (Fe), and zinc (Zn)] in the blood, liver, kidney, brain, and spleen of Wistar rats and to determine the protective potential of selenium (Se) against fluoride (F-) toxicity. Male Wistar rats were randomly distributed in groups of five (n=5) receiving tap water (control) or water with NaF 150 mg/L, NaF 150 mg/L + Se 1.5 mg/L, and Se 1.5 mg/L solutions ad libitum for 28 days. Fluorides caused an imbalance in the redox and biometal (Cu, Fe, and Zn) status, leading to high superoxide anion (O2 .-) and malondialdehyde (MDA) levels in the blood and brain and a drop in superoxide dismutase (SOD1) activity in the liver and its increase in the brain and kidneys. Se given with NaF improved MDA, SOD1, and O2 .- in the blood, brain, and kidneys, while alone it decreased SH group levels in the liver and kidney. Biometals both reduced and increased F- toxicity. Further research is needed before Se should be considered as a promising strategy for mitigating F- toxicity.

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