Effects and Characterization of Some Topical Ointments Based on Vegetal Extracts on Incision, Excision, and Thermal Wound Models
Calin Vasile Andritoiu,
Corina Elena Andriescu,
Constanta Ibanescu,
Cristina Lungu,
Bianca Ivanescu,
Laurian Vlase,
Cornel Havarneanu,
Marcel Popa
Affiliations
Calin Vasile Andritoiu
Apitherapy Medical Center, Balaneşti, 217036 Gorj, Romania
Corina Elena Andriescu
Department of Morphofunctional Sciences I—Histology, Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania
Constanta Ibanescu
Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iasi, Prof. D. Mangeron Blvd., 700050 Iasi, Romania
Cristina Lungu
Department of Pharmaceutical Botany, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania
Bianca Ivanescu
Department of Pharmaceutical Botany, Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania
Laurian Vlase
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, 8 Victor Babes Street, 40010 Cluj-Napoca, Romania
Cornel Havarneanu
Faculty of Psychology and Education Sciences, Alexandru Ioan Cuza University, 700554 Iasi, Romania
Marcel Popa
Department of Natural and Synthetic Polymers, Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iasi, Prof. D. Mangeron Blvd., 700050 Iasi, Romania
The present paper aims to formulate and characterize four phytotherapeutic ointments based on Hippophae fructus, Calendulae flos, Bardanae folium, and Millefolii herba, which are included in a novel ointment base. In order to investigate the healing properties of the ointments, in vivo experimental wound models of linear incision, circular excision, and thermal burn were performed on Wistar rats. Topical treatment was performed daily for 21 days. Determination of the wound contraction rate (WCR), the period of reepithelization, and histopathological examination were achieved. Additionally, for the tested ointments, oscillatory and rotational rheological tests were carried out, and for the extracts, HPLC analysis was performed. The results demonstrate that the tested novel ointments are safe for use and the most effective ointment proved to be the one based on Arctium lappa, followed by that of Calendulae flos.
