DH-plant production in culture of unpollinated ovules of cucumber (Cucumis sativus L.)

Abstract

Relevance. The development of F1 hybrids distinguishing it from cultivars by high productivity, plant uniformity in ripening date, fruit sizes and quality is the promising trend in breeding program in cucumber (Cucumis sativus L.).The aim of the study was to optimize the gynogenesis induction condition in culture of unpollinated ovules in vitro in order to broad the generation of new breeding forms and to accelerate homozygous line production.Materials and methods. Eight promising cucumber accessions from Laboratory of Cucurbit Breeding and Seed Production (FSBSI FSVC) were taken for the study. The protocol developed in Laboratory of Biotechnology (FSBSI FSVC) for production of doubled haploid in Cucurbitaceae family was used in the experiment. The medium IMC with 30 g/L sucrose and 7g/L agar supplemented with 200 mg/L ampicillin and 0.2 mg/L thidiazuron (TDZ) was applied to induce gynogenic development.Results. The half-open bud or flower was shown to be the most suitable to be taken as an explant for cultivation. Highest number of embryo-like structures in all accessions developed from ovaries 2.1-2.6 cm long. Exposure to sterilization solution of sodium hypochlorite for 15 min made ovary wall softer and ovules can be then easily extracted without traumatizing. The traumatized ovule resulted in inhibited gynogenic development. Embryoids and calli had developed in all studied cucumber accessions, but well-formed plants were only obtained in six accessions. In total 26 plants were produced. The maximum gynogenesis induction equal to 63.1% was achieved in accession 1810. Maximum number of plant produced was twelve in accession 1763, but the greatest plant outcome 7.7% of the ovules with induced gynogenesis was observed in accession 1807.

Keywords

• cucumber
• cucumis sativus l.
• dh-plants
• culture of unpollinated ovules in vitro
• gynogenesis
• haploids of cucumber
• homozygous lines