Kinetic parameters of sperm in fresh and cryopreserved semen of alpaca (Vicugna pacos L.)

Abstract

Cryopreservation of alpaca sperm is important for genetic improvement. The purpose of this work was to evaluate the sperm kinetic parameters in fresh semen and cryopreserved in dilutors, using the CASA team. 3 male alpacas were used, which were trained for a month in the semen collection with the artificial vagina. The sperm was frozen using two diluents (commercial AndroMed) and (commercial AndroMed, citric acid, gentamicin and glycerol). The semen had the following characteristics, volume 1.5±0.7 mL, viscosity 5.9±1.5, pH 7.1, with an alkalinity tendency and the color that predominated was the translucent white. The averages of kinetic parameters found in fresh semen were VCL (25.8±4.2 µm/s), VSL (10.3±2.5 µm/s) and VAP (18.9±3.2 µm/s). Kinetic parameters in cryopreserved semen showed that dilutor 2 VCL (8.4±1.4 µm/s), dilutor 1 (7.2±1.2 µm/s), VSL dilutor 2 (3.4±0.8 µm/s), dilutor 1 (2.9±0.7µm/s), VAP dilutor 2 (5.8±1.0 µm/s), dilutor 1 (4.8±0.8 µm/s). It is concluded that dilutor 2 is another alternative for cryopreservation of alpaca semen.

Keywords

• semen
• casa
• evaluation
• cryopreservation
• alpaca
• kinetic parameters.