Desflurane 6% inhalation inhibits erythrocyte deformability and alters oxidative stress in rat lung and kidney in a time-dependent manner

Abstract

Time-dependent effects of 6% desflurane applied for 6 h on erythrocyte deformability and tissue oxidative stress levels within 24 h period are unknown. This study aimed to investigate the influences of 6% desflurane, on erythrocyte deformability and serum, heart, lung, kidney oxidative stress in a time-dependent manner (6 h intervals) following exposure. 10-12 week-old Wistar-albino male rats were divided into five groups (n=6 per group). Rats of the 0h group were not exposed desflurane. The other rats were named in terms of time (6 h, 12 h, 18 h, 24 h) passed following anesthesia. Desflurane was administered at concentration of 6% in 6L min−1 flow-rate of 100% oxygen for 6 h in an anesthetic chamber. Erythrocyte deformability was determined using an ectacytometer. Total oxidant status (TOS), total antioxidant status (TAS) were determined using commercial kits, while oxidative stress index (OSI) was calculated. Desflurane inhalation caused a general decrement in erythrocyte deformability, the effect being more prominent at 18th h following anesthesia. Oxidative stress was not altered in serum, heart. TOS was increased at 6th and 18th h following desflurane exposure in lung. TOS and OSI in kidney were decreased at 18th and 24th h compared to 6th. Our results suggest that the oxidative potential and adverse effect of deflurane on tissue oxygenation by inhibiting RBC deformability in the first 24 h should be kept in mind especially in the presence of comorbidities.

Keywords

• desflurane
• erythrocyte deformability
• oxidative stress
• lung
• kidney