Structural and biochemical insights into small RNA 3′ end trimming by Arabidopsis SDN1

Jiayi Chen; Li Liu; Chenjiang You; Jiaqi Gu; Wenjie Ruan; Lu Zhang; Jianhua Gan; Chunyang Cao; Ying Huang; Xuemei Chen; Jinbiao Ma

doi:10.1038/s41467-018-05942-7

Nature Communications (Sep 2018)

Structural and biochemical insights into small RNA 3′ end trimming by Arabidopsis SDN1

Jiayi Chen,
Li Liu,
Chenjiang You,
Jiaqi Gu,
Wenjie Ruan,
Lu Zhang,
Jianhua Gan,
Chunyang Cao,
Ying Huang,
Xuemei Chen,
Jinbiao Ma

Affiliations

Jiayi Chen: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Li Liu: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California
Chenjiang You: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California
Jiaqi Gu: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Wenjie Ruan: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Lu Zhang: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
Jianhua Gan: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
Chunyang Cao: State Key Laboratory of Bio-organic and Natural Product Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
Ying Huang: State Key Laboratory of Molecular Biology, National Center for Protein Science Shanghai, Shanghai Science Research Center, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences
Xuemei Chen: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California
Jinbiao Ma: State Key Laboratory of Genetic Engineering, Collaborative Innovation Centre of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University

DOI: https://doi.org/10.1038/s41467-018-05942-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

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Small RNA degrading nucleases (SDNs) can degrade short RNAs. Here the authors report the crystal structure of Arabidopsis SDN1 in complex with a single-stranded RNA, and provide new insight into 3′ end trimming mechanism of 3′ to 5′ riboexonucleases in the metabolism of various species of small RNAs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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