Aptamer-based Colorimetric and Chemiluminescence Detection of Aflatoxin B1 in Foods Samples

Abstract

We developed colorimetric and chemiluminescence methods for the detection of aflatoxin B1(AFB1) based on the interaction of gold nanoparticles (AuNPs) with the aptamer. Aggregation of AuNPs was induced by desorption of the AFB1 binding aptamer from the surface of AuNPs as a result of the aptamer target interaction leading to the color change of AuNPs from red to purple. The linear range of the colorimetric aptasensor covered a large variation of AFB1 concentration from 80 to 270 nM and the detection limit of 7 nM was obtained. Also, the catalytic activity of the aggregated AuNPs was greatly enhanced by chemiluminescence (CL) reaction, where the detection limit was enhanced to 0.5 nM with a regression coefficient of R2 = 0.9921. Here we showed that the sensitivity of detection was increased by employing CL and using the catalytic activity of aggregated AuNPs, on the luminol–hydrogen peroxide reaction. Therefore the proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, providing a viable alternative for rapid screening of toxin in foods.

Keywords

• Aptamer
• colorimetric
• chemiluminescence
• aflatoxin B1