We report new northwesternmost records of the Northern Little Yellow-eared Bat, Vampyressa thyone Thomas, 1909 (Phyllostomidae, Stenodermatinae), from Sierra de Atoyac, Guerrero, Mexico. We identified four individuals using morphological characters; two of the individuals were collected at 1,234 and 1,285 m above sea level, altitudes atypical for this species. Captures were recorded between 19:20 and 23:18 h, and all individuals presented signs of reproductive activity. With these data, we increase the scarce knowledge about this rare frugivorous bat.