Fabrication and characterization of silver nanorods incorporated calcium silicate scaffold using polymeric sponge replica technique

Pawan Kumar; Brijnandan S. Dehiya; Anil Sindhu; Ravinder Kumar; Catalin I. Pruncu; Anil Yadav

Materials & Design (Oct 2020)

Fabrication and characterization of silver nanorods incorporated calcium silicate scaffold using polymeric sponge replica technique

Pawan Kumar,
Brijnandan S. Dehiya,
Anil Sindhu,
Ravinder Kumar,
Catalin I. Pruncu,
Anil Yadav

Affiliations

Pawan Kumar: Department of Materials Science and Nanotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India; Corresponding authors.
Brijnandan S. Dehiya: Department of Materials Science and Nanotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India
Anil Sindhu: Department of Biotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India
Ravinder Kumar: School of Mechanical Engineering, Lovely Professional University, Phagwara 144411, India; Corresponding authors.
Catalin I. Pruncu: Mechanical Engineering Department, University of Birmingham, Birmingham B15 2TT, UK; Mechanical Engineering, Imperial College London, London SW7 2AZ, UK; Corresponding author at: Mechanical Engineering Department, University of Birmingham, Birmingham B15 2TT, UK.
Anil Yadav: Department of Chemical Engineering, Deenbandhu Chhotu Ram University of Science and Technology, Murthal, 131039, India

Journal volume & issue: Vol. 195
p. 109026

Abstract

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The risk of infection in bones after surgical procedures can be reduced by using antibacterial scaffolds. This study was aimed at preparing a silver nanorods (Ag-nr) incorporated wollastonite (CaSiO3) scaffold using the polymeric sponge replica method. The powdered Ag-nr sample shows an ultrafine microstructure with a minimum rod diameter of 20.8 nm. The Ag nanorods were fabricated by a reflux method and the sol-gel derived Ag-incorporated-CaSiO3 samples (Ag-CaSiO3) were characterized through FTIR, XRD, TEM, SEM, and BET analytical techniques. The electron microscopic results of the scaffold revealed a well-defined porous construction with pore sizes varying from 103 to 164 μm which are suitable for cell infiltration. The Ag-CaSiO3 scaffold showed a brittle behavior with compressive strength of 11.7 MPa. The in-vitro degradation and mineralization behavior of the Ag-CaSiO3 scaffold was investigated using SBF. The Ag-CaSiO3 samples present sound antibacterial effects against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella typhi without negotiating the formation of apatite layer in simulated body fluid (SBF). The in-vitro cell viability and attachment studies with MG-63 cells using Alamar blue assay confirmed the cytocompatible nature of Ag-CaSiO3. The rationally designed Ag-CaSiO3 sample can generate a healthier substitute for bone tissue engineering compared to other similar materials.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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