EXPRESSION OF THE GROWTH FACTOR RECEPTOR-BOUND PROTEIN-2 GENE IN RENAL CARCINOMA AND ITS ASSOCIATION WITH PATIENT PROGNOSIS BASED ON GENE DATABASE ANALYSIS

Abstract

Objective To investigate the expression of the growth factor receptor-bound protein-2 (GRB2) gene in renal carcinoma and its influence on prognosis. Methods The ONCOMINE database was used to obtain the data on the expression of the GRB2 gene in renal carcinoma, and the TCGA database was used to analyze the difference in the expression of the GRB2 gene between renal carcinoma tissue and normal tissue, as well as the association between the expression of the GRB2 gene and the prognosis of patients. The STRING database was used to construct the protein-protein interaction network of the GRB2 gene. Quantitative real-time PCR was used to measure the relative expression level of the GRB2 gene in six renal carcinoma tissue samples and corresponding adjacent normal tissue samples. Results The expression of the GRB2 gene in renal carcinoma tissue was significantly higher than that in normal renal tissue (t=8.21,P<0.05), and the increased expression of the GRB2 gene in renal carcinoma tissue was associated with the prognosis of renal carcinoma (z=2.71,P<0.05). Further analysis of the 14 GRB2-related interacting proteins showed that GRB2 was mainly involved in cell proliferation and apoptosis. The relative expression level of the GRB2 gene in renal carcinoma tissue was significantly higher than that in the corresponding adjacent normal tissue (t=6.98,P<0.001). Conclusion The GRB2 gene is highly expressed in renal carcinoma tissue, and its expression level is significantly asso-ciated with the prognosis of patients with renal carcinoma.

Keywords

• kidney neoplasms|grb2 adaptor protein|prognosis|gene expression profiling|protein interaction maps|databases, genetic|data mining