Identification and expression analysis of sex biased miRNAs in chinese hook snout carp Opsariichthys bidens

Rongkang Tang; Rongkang Tang; Cong Xu; Cong Xu; Yefei Zhu; Yefei Zhu; Jinpeng Yan; Ziliang Yao; Wenzong Zhou; Lang Gui; Lang Gui; Mingyou Li; Mingyou Li

doi:10.3389/fgene.2022.990683

Frontiers in Genetics (Sep 2022)

Identification and expression analysis of sex biased miRNAs in chinese hook snout carp Opsariichthys bidens

Rongkang Tang,
Rongkang Tang,
Cong Xu,
Cong Xu,
Yefei Zhu,
Yefei Zhu,
Jinpeng Yan,
Ziliang Yao,
Wenzong Zhou,
Lang Gui,
Lang Gui,
Mingyou Li,
Mingyou Li

Affiliations

Rongkang Tang: Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China
Rongkang Tang: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China
Cong Xu: Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China
Cong Xu: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China
Yefei Zhu: Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China
Yefei Zhu: Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
Jinpeng Yan: Department of Cell Biology, School of Life Sciences, Central South University, Changsha, China
Ziliang Yao: Lishui Fishery Technical Extension Station, Lishui, Zhejiang, China
Wenzong Zhou: Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
Lang Gui: Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China
Lang Gui: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China
Mingyou Li: Key Laboratory of Integrated Rice-fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China
Mingyou Li: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China

DOI: https://doi.org/10.3389/fgene.2022.990683
Journal volume & issue: Vol. 13

Abstract

Read online

As an economically important fish, Opsariichthys bidens has obvious sexual dimorphism and strong reproductive capacity, but no epigenetics study can well explain its phenotypic variations. In recent years, many microRNAs involved in the regulation of reproductive development have been explored. In this study, the small RNA libraries of O. bidens on the testis and ovary were constructed and sequenced. A total of 295 known miRNAs were obtained and 100 novel miRNAs were predicted. By comparing testis and ovary libraries, 115 differentially expressed (DE) miRNAs were selected, of which 53 were up-regulated and 62 were down-regulated. A total of 64 GO items (padj < 0.01) and 206 KEGG pathways (padj < 0.01) were enriched in the target gene of miRNA. After that, the expression levels of nine DE miRNAs, including let-7a, miR-146b, miR-18c, miR-202-5p, miR-135c, miR-9-5p, miR-34c-3p, miR-460-5p and miR-338 were verified by qRT-PCR. Furthermore, bidirectional prediction of DE miRNAs and sex-related genes was carried out and the targeting correlation between miR-9-5p and nanos1 was verified by Dual-Luciferase reporter assay. Our findings identified the differentially expressed miRNA and paved the way to new possibilities for the follow-up study on the mechanism of miRNA-mRNA interaction in the gonads of O. bidens.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

About the journal

Abstract

Keywords