Damage to Liver Tissue Caused by Valproic Acid Used for Treating Epilepsy: Protective Effects of Vitamin B6

Abstract

Objective:We intended to determine the vitamin B6 (Vit B6) protection on valproic acid (VPA)-induced liver injury.Methods:Male Sprague-Dawley rats were used. The control group: Vit B6 (50 mg/kg/day) given rats; VPA (500 mg/kg/day) given rats; VPA and Vit B6 given rats at the same dose and time for 7 days.Results:Liver glutathione and total antioxidant capacity were decreased while, lipid peroxidation, advanced oxidized protein products, tumor necrosis factor-alpha, interleukin-6, nitric oxide, total oxidant status, oxidative stress index, reactive oxygen species levels and myeloperoxidase, antioxidant enzyme activities, glucose-6-phosphate dehydrogenase, and adenosine deaminase activities were increased in the VPA group. Vit B6 eased these parameters in the VPA group. In the histological determinations, nuclei including dense chromatin material, hyperemia, sinusoidal dilation, collagen accumulation in connective tissue, and large and dense granules in the cytoplasm were increased in the VPA group according to the control groups, microscopically.Conclusion:As a result, Vit B6 supplementation reversed biochemical results in VPA-induced liver damage by regulating the antioxidant status.

Keywords

• valproic acid
• vitamin b6
• hepatotoxicity
• oxidative stress
• antioxidant effect