Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke Opisthorchis viverrini infection
Krongkarn Watakulsin,
Arpa Surapaitoon,
Lorina Handayani Ulag,
Sunheng Kaing,
Watcharapol Suyapoh,
Prasert Saichua,
Kanin Salao,
Sirikachorn Tangkawatana,
Sutas Suttiprapa
Affiliations
Krongkarn Watakulsin
Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Arpa Surapaitoon
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Lorina Handayani Ulag
Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta 13210, Indonesia
Sunheng Kaing
Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Watcharapol Suyapoh
Faculty of Veterinary Science, Prince of Songkla University, Songkhla 90110, Thailand
Prasert Saichua
Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Kanin Salao
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Sirikachorn Tangkawatana
Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Department of Tropical Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Tropical Disease Research Center, WHO Collaborating Centre for Research and Control of Opisthorchiasis, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Opisthorchis viverrini is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against O. viverrini infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host.
