The Effects of Plant Extracts Mixture on Sperm Parameters and Damaged Testicular Tissue With Carbon Tetrachloride in Mice

Abstract

Introduction: Spermatogenesis is a process in which sperm is produced, and its disruption at any stage can lead to infertility. Plant extracts have strong phytochemicals, like s anthocyanin. Applying decoction of this plant’s leaves could relieve nausea, and its roots are used to treat dysentery. Methods: The Naval Medical Research Institute (NMRI) mice were used and grouped into two control and treatment groups. The control group received distilled water and, the treatment group was fed with 250 mg/kg.bw mixture of plants daily after the disruption with Carbon Tetrachloride (CCl4) for 60 days. After this period, the mice got unconscious, and their testicles were removed from the abdomen. After conducting the morphologic study, including measuring the samples’ dimensions and weight, their testicles were transcended and stained with eosin hematoxylin method. All data were analyzed by SPSS V. 22. The significance level was set at P<0.05. Results: The study results revealed significant differences between the testicular size and weight. Moreover, the number of spermatogonia, spermatocytes, spermatozoa, and Leydig cells increased in the experimental group, compared to the controls (P<0.01). Conclusion: The mixture of the plant caused a significant increase in spermatogenesis cells in male mice and increased their fertility.

Keywords

• mice
• spermatogenesis
• infertility
• extract