Probiotics reduce anxiety-related behavior in zebrafish
David G. Valcarce,
Juan M. Martínez-Vázquez,
Marta F. Riesco,
Vanesa Robles
Affiliations
David G. Valcarce
IEO, Spanish Institute of Oceanography, Planta de Cultivos El Bocal, Barrio Corbanera, Monte, 39012, Santander, Spain
Juan M. Martínez-Vázquez
IEO, Spanish Institute of Oceanography, Planta de Cultivos El Bocal, Barrio Corbanera, Monte, 39012, Santander, Spain
Marta F. Riesco
IEO, Spanish Institute of Oceanography, Planta de Cultivos El Bocal, Barrio Corbanera, Monte, 39012, Santander, Spain
Vanesa Robles
IEO, Spanish Institute of Oceanography, Planta de Cultivos El Bocal, Barrio Corbanera, Monte, 39012, Santander, Spain; Cell Biology Area, Department of Molecular Biology, Universidad de León, 24071, León, Spain; Corresponding author.
There is increasing evidence that gut microbiome could have effects on neurological processes and on behavior. In this study we used the novel tank test (NTT) to analyze zebrafish exploring behavior after four months’ supplementation with probiotics with probed antioxidant and anti-inflammatory properties. Results showed that prolonged ingestion of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 significantly alters the swimming pattern and mean swimming speed in the zebrafish model. After treatment, zebrafish strongly reduced their bottom-dwelling geotactic behavior when placed in a new tank, which could be correlated to a lower state of anxiety.
