Formulation and Characterization of Doxycycline-Loaded Polymeric Nanoparticles for Testing Antitumor/Antiangiogenic Action in Experimental Colon Cancer in Mice
Reem Alshaman,
Abdullah Alattar,
Rehab M. El-Sayed,
Ahmed R. Gardouh,
Rabie E. Elshaer,
Amany Y. Elkazaz,
Mohamed Ahmed Eladl,
Mohamed El-Sherbiny,
Noha E. Farag,
Ahmed Mohsen Hamdan,
Sawsan A. Zaitone
Affiliations
Reem Alshaman
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia
Abdullah Alattar
Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia
Rehab M. El-Sayed
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Sinai University, El-Arish 45511, Egypt
Ahmed R. Gardouh
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
Rabie E. Elshaer
Department of Pathology, Faculty of Medicine, Al-Azhar University, Cairo 11651, Egypt
Amany Y. Elkazaz
Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
Mohamed Ahmed Eladl
Basic Medical Science Department, University of Sharjah, Sharjah 27272, United Arab Emirates
Mohamed El-Sherbiny
Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh 71666, Saudi Arabia
Noha E. Farag
Department of Physiology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
Ahmed Mohsen Hamdan
Department of Pharmacy Practice, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia
Sawsan A. Zaitone
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
Nanotherapeutics can enhance the characteristics of drugs, such as rapid systemic clearance and systemic toxicities. Polymeric nanoparticles (PRNPs) depend on dispersion of a drug in an amorphous state in a polymer matrix. PRNPs are capable of delivering drugs and improving their safety. The primary goal of this study is to formulate doxycycline-loaded PRNPs by applying the nanoprecipitation method. Eudragit S100 (ES100) (for DOX-PRNP1) and hydroxypropyl methyl cellulose phthalate HP55 (for DOX-PRNP2) were tested as the drug carrying polymers and the DOX-PRNP2 showed better characteristics and drug release % and was hence selected to be tested in the biological study. Six different experimental groups were formed from sixty male albino mice. 1,2,-Dimethylhydrazine was used for 16 weeks to induce experimental colon cancer. We compared the oral administration of DOX-PRNP2 in doses of 5 and 10 mg/kg with the free drug. Results indicated that DOX-PRNP2 had greater antitumor activity, as evidenced by an improved histopathological picture for colon specimens as well as a decrease in the tumor scores. In addition, when compared to free DOX, the DOX-PRNP2 reduced the angiogenic indicators VEGD and CD31 to a greater extent. Collectively, the findings demonstrated that formulating DOX in PRNPs was useful in enhancing antitumor activity and can be used in other models of cancers to verify their efficacy and compatibility with our study.
