Directing curli polymerization with DNA origami nucleators

Xiuhai Mao; Ke Li; Mengmeng Liu; Xinyu Wang; Tianxin Zhao; Bolin An; Mengkui Cui; Yingfeng Li; Jiahua Pu; Jiang Li; Lihua Wang; Timothy K. Lu; Chunhai Fan; Chao Zhong

doi:10.1038/s41467-019-09369-6

Nature Communications (Mar 2019)

Directing curli polymerization with DNA origami nucleators

Xiuhai Mao,
Ke Li,
Mengmeng Liu,
Xinyu Wang,
Tianxin Zhao,
Bolin An,
Mengkui Cui,
Yingfeng Li,
Jiahua Pu,
Jiang Li,
Lihua Wang,
Timothy K. Lu,
Chunhai Fan,
Chao Zhong

Affiliations

Xiuhai Mao: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Ke Li: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Mengmeng Liu: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University
Xinyu Wang: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Tianxin Zhao: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Bolin An: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Mengkui Cui: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Yingfeng Li: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Jiahua Pu: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University
Jiang Li: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Lihua Wang: Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Timothy K. Lu: Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology
Chunhai Fan: School of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Chao Zhong: Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University

DOI: https://doi.org/10.1038/s41467-019-09369-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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Curli are bacterial functional amyloids that have gained interest as self-assembling biomaterial for biotechnology applications. Here, the authors show that DNA origami decorated with CsgB nucleator proteins induced the site-specific nucleation and subsequent fibrillization of CsgA proteins.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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