An FTIR Spectroscopy Investigation on Different Methods of Lipid Extraction from HepG2 Cells

Abstract

Fourier transform infrared (FTIR) spectroscopy is a non-invasive technique that is largely used for studying lipidomics. Lipids are a primary class of biological molecules that play numerous vital roles in various processes. In the present work, we adopted FTIR spectroscopy for monitoring the lipid extraction efficiency of different methods used for extracting lipids from hepatocarcinoma cells. The spectra acquired from samples obtained with the selected methods showed the contributions of different functional groups. A qualitative comparison indicated that all the spectra exhibited similar lipid species profiles. The peak intensity attributed to the CH2 asymmetric stretching mode has been used for a quantitative comparison of the efficiency of the different extraction methods.

Keywords

• lipid extraction methods
• FTIR spectroscopy
• HepG2 cells