Microenvironment-triggered cascade metal-polyphenolic nanozyme for ROS/NO synergistic hyperglycemic wound healing
Shuo Shi,
Yaru Han,
Jianxing Feng,
Jingru Shi,
Xiaoling Liu,
Bangfeng Fu,
Jianlong Wang,
Wentao Zhang,
Jinyou Duan
Affiliations
Shuo Shi
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China; College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
Yaru Han
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China; Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA
Jianxing Feng
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
Jingru Shi
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China
Xiaoling Liu
Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA
Bangfeng Fu
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China
Jianlong Wang
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China; Corresponding author.
Wentao Zhang
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China; Corresponding author.
Jinyou Duan
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi, China; Corresponding author.
Wound infection of hyperglycemic patient often has extended healing period and increased probability due to the high glucose level. However, achieving precise and safe therapy of the hyperglycemic wound with specific wound microenvironment (WME) remains a major challenge. Herein, a WME-activated smart L-Arg/GOx@TA-Fe (LGTF) nanozymatic system composed of generally recognized as safe (GRAS) compound is engineered. The nanozymatic system combining metal-polyphenol nanozyme (tannic acid-Fe3+, TA-Fe) and natural enzyme (glucose oxidase, GOx) can consume the high-concentration glucose, generating reactive oxygen species (ROS) and nitric oxide (NO) in situ to synergistically disinfect hyperglycemia wound. In addition, glucose consumption and gluconic acid generation can lower glucose level to promote wound healing and reduce the pH of WME to enhance the catalytic activities of the LGTF nanozymatic system. Thereby, low-dose LGTF can perform remarkable synergistic disinfection and healing effect towards hyperglycemic wound. The superior biosafety, high catalytic antibacterial and beneficial WME regulating capacity demonstrate this benign GRAS nanozymatic system is a promising therapeutic agent for hyperglycemic wound.
