The influence of pore geometry in cp Ti-implants - a cell culture investigation

Abstract

Biocompatibility testing of differently structured titanium implants was performed using an in vitro test system of a newly established human fetal osteoblastic cell line (hFOB 1.19). Cell adhesion of osteoblastic cells on the different porous geometries and the suitability of a copper vapor laser system for surface structuring was tested with the following parameters: cell-number, cell viability, alkaline phosphatase expression. The analysis of the cell culture results demonstrated that 25µm and 200µm porous geometries showed similar or even better results than the negative control of polystyrene; there was no sign of toxic effects. However, the 100µm porous geometry showed an impressive negative influence on the calculated parameters. The reason for this effect is unclear. The series with 50µm, 300µm, 400µm and 500µm showed a comparable, intermediate effect on the cell culture with respect to the different parameters. However, the results were worse than with the 25 and 200µm porous geometry. In conclusion, the 25µm and 200µm porous geometry seems to have the most positive effect on the human osteoblastic cell line hFOB 1.19.