The Comparison of Juvenile Hormone and Transcriptional Changes between Three Different Juvenile Hormone Analogs Insecticides on Honey Bee Worker Larval’s Development
Jie Luo,
Sheng Liu,
Jiangan Hou,
Lichao Chen,
Honghong Li,
Shucheng Liao,
Qimei Tan,
Taiyi Yang,
Guoqiang Yi,
Fu Zhang,
Xuesheng Li
Affiliations
Jie Luo
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Sheng Liu
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Jiangan Hou
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Lichao Chen
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Honghong Li
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Shucheng Liao
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Qimei Tan
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Taiyi Yang
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Guoqiang Yi
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Fu Zhang
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Xuesheng Li
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, Guangxi University, Nanning, Guangxi 530004, China
Juvenile hormones (JHs) play a crucial role in the development of honey bee (Apis mellifera) worker larvae. Juvenile hormone analogs (JHAs), insecticides widely used in pest control, have been reported to affect the health and survival of honey bee worker larvae. However, the molecular mechanisms of JHAs in the honey bee remain unclear. In this study, we treated honey bee worker larvae with pyriproxyfen, fenoxycarb, and methoprene, three different JHAs. We monitored the changes in the transcription of genes encoding major JH response enzymes (CYP15A1, CYP6AS5, JHAMT, and CHT1) using RT-qPCR and analyzed the transcriptome changes in worker larvae under JHA stress using RNA-seq. We found that the enrichment pathways differed among the treatment groups, but the classification of each pathway was generally the same, and fenoxycarb affected more genes and more pathways than did the other two JHAs. Notably, treatment with different JHAs in the honey bee changed the JH titers in the insect to various extents. These results represent the first assessment of the effects of three different JHAs on honey bee larvae and provide a new perspective and molecular basis for the research of JH regulation and JHA toxicity in the honey bee.
