OPTIMIZATION OF HPLC METHODS FOR QUANTITATIVE DETERMINATION OF GLYCYRRHIZIC ACID IN LICORICE ROOTS

Abstract

Comparative evaluation of glycyrrhizic acid content in licorice roots by methods of UV-spectrophotometry (GF RF XIII) and high-performance liquid chromatography (HPLC) was carried out. Sample preparation for HPLC method of quantitative determination of glycyrrhizic acid in samples of domestic production is developed. Small selectivity of UV-spectrophotometry at determination of glycyrrhizic acid is experimentally established and perspective of HPLC method for standardization of licorice raw materials is shown.

Keywords

• glycyrrhizic acid
• licorice raw materials
• monoammonium glycyrrhizinate
• high-performance liquid chromatography (hplc)
• uv spectrophotometry
• sample preparation
• selectivity of the method
• quantitative determination
• medicinal plant raw materials