Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

Abstract

A high-throughput screening protocol for evaluating chimeric, self-sufficient P450 biocatalysts and their mutants against a panel of substrates was developed, leading to the identification of a number of novel biooxidation activities.

Keywords

• biocatalysis
• C–H activation
• high-throughput screening
• P450 monooxygenase
• substrate engineering